A fluorometer is an instrument that uses optical filters to isolate incident light and fluorescent light, and measures the sample's ability to absorb light at one wavelength and emit light at a longer wavelength.
In these devices, a radiation source (luminous) emits light of an excitation wavelength for the compound to be measured or analyzed. The excitation of the compound to be analyzed is produced through the use of optical filters, these work by blocking other wavelengths and transmitting the appropriate wavelength to produce excitation of the compound. The light generated by the radiation source passes through the sample to be measured, and a certain amount of wavelength is absorbed, while it is then emitted at a longer wavelength. This emitted light is measured by a detector.
Fluorometers are highly sensitive, and the optical filters used by them are generally inexpensive and easy to change. This makes fluorometers widely used equipment in scientific and experimental research, and for academic purposes.
A fluorometer is a piece of equipment that allows the analysis, identification and quantification of chemical substances with molecules capable of fluorescence, using the technique of fluorescence spectrophotometry or fluorometry. We can say that this equipment is a special optical device used in laboratories, capable of measuring the fluorescent quality of biological or mineral samples.
Fluorescence occurs when a substance emits visible light and glows after it has been exposed to some type of radiation, either alone or high-energy radiation such as X-rays from visible light. This property is similar to phosphorescence, which is a low-temperature emission of light by an accumulation of energy or radiation from a substance. Fluorescence analysis is an analytical method related to spectrophotometry, since many molecules are capable of emitting this energy as radiation, with which they return to the ground state, the radiation emitted is known as fluorescence.
A fluorometer measures the parameters of fluorescence its intensity and wavelength distribution of the emission spectrum after excitation by a certain light spectrum. Applications include chemistry/biochemistry, medicine, environmental monitoring. That means they are used to measure chlorophyll fluorescence to investigate plant physiology. These are key devices in any biological laboratory, especially for research. That is why we present you with this high-tech device, to turn your laboratory into a reliable company.
YR412-A is your solution, as it is capable of measuring the absorbance of all ingredients (such as DNA, RNA, oligos, etc.) in the sample at 260nm, but be aware because that may make the reading inaccurate. The fluorometer measures the fluorescence intensity of the fluorescent reagent, which combines with the target molecule to read the concentration of the sample.
Each model that we, KALSTEIN, as a Laboratory Equipment company, offers you, has different settings, parts and characteristics that range from the concept of different refrigeration and freezing temperatures to design, you can see it through our online purchase channels
The detection of a fluorometer is based on the fundamental universal principles of fluorescence, there are several unique applications and adaptations for these devices. One of the main uses of fluorometers is that they are used to measure the fluorescence of chlorophyll and thus investigate the physiology of plants.
These equipments are also used in biochemical, medical, chemical and research analysis of organic compounds. Currently one of the most recent applications of fluorometry is the quantification of nucleic acids, through fluorometers, which measure concentrations of DNA, RNA, and proteins with high precision and sensitivity.
It is based on the use of fluorophores that are specifically intercalated between the molecules of interest, thus minimizing the effects of contaminants. The precision of the measurements even at low concentrations makes these kits an ideal tool for applications such as real-time PCR and massive sequencing.
There are countless models, so it is normal that you do not know which laboratory equipment to buy that suits your needs. At Kalstein, we evaluate them to find what you're looking for.
A fluorometer is a piece of equipment that allows the analysis, identification and quantification of chemical substances with molecules capable of fluorescing, using the technique of fluorescence spectrophotometry or fluorometry.
A fluorometer is a laboratory instrument that allows the analysis, identification and quantification of chemical substances with molecules capable of fluorescing, using the technique of fluorescence spectrophotometry ...
There are two general instruments to measure fluorescence, which are fluorometers and spectrofluorometers, in this article we will focus on the differences between both teams.Fluorescence can be defined as a type of ...
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