Lichen planus is a benign disease with clinical and histopathological findings. It has a clear result on epithelial transition and immune cell function. And it is detected with assays such as indirect immunoflorescence Elisa that is detected with circulating antibodies in the serum that bind to collagen domains.
In addition, and according to the functions performed by the microplate washer, it will allow the detection of this disease, since it is a team responsible for keeping clean the wells where samples are deposited and decreases the background character caused by unbound antibodies, which would cause the increase in the crossings of the analysis.
Therefore, with the Kalstein brand microplate washer, it ensures that only specific bonding mixtures are retained, generating transmissions in the final step. It does not allow improper washing and prevents discrepancies, thanks to its adjustable and programmable washing modes.
Use of the Microplate Washer in Lichen Planus
To demonstrate the degree of danger to the health of patients in the determination of diseases such as lichen planus, the Elisa trials are part of such practices, since it has been shown that saliva can be used as an alternative to serum for the purposes of these techniques and monitoring of the disease. Therefore, with this study technique accompanied with the microplate washer, it is able to demonstrate that options for symptomatic treatment, monitoring the problem and finding the problem of malignant transformation can be addressed.
According to the importance of the microplate washer, the processes of cleaning the wells containing the samples and a series of measures that affect the activity of the washing step of an Elisa plate are involved. These measures involve suction and the position of the suction where they can be adjusted to decrease residual volume and transitions.
Residual substances have free antibodies that enlarge the background indication, but the smaller the volume of the substances is better, so that it does not interfere with the next step. Likewise, the height of the suction can cause the residual volume, because if the distance between the cleaning tip and the bottom of the reaction well is greater, it will be enlarged in an energetic way.
Generally, the microplate washer uses two heads for washing processes, they are rigid and floating. The floating suction head can be easily shaken up and down during the processes, while the rigid one is more difficult to improve, and fits at a height suitable for suction.
The position of the suction is also an important characteristic, since its effect is very important on the residual volume, using a single suction point per well, it is optimized and improves the function. The location depends on the structure of the head and is hardly located in the middle of the reaction well, although it is the most common default location for all washes.
Another important point concerns the shape of wells as it affects the amount of residual substances. The C-shaped microplate of flat backgrounds with rounded edges are usually indicated, because they support precise amounts than those with flat backgrounds and sharp corners.
Microplate Washing Machine Brand Kalstein
With the use of our Microplate Washing Machine for the practice of Elisa tests, belonging to the YR series offered by Kalstein, meets soaking and vibration time programs, in addition to having a power supply of 220 – 110 VAC ± 10%, 50 60Hz 100W. Automatic waste and distilled water liquid lotion and warning. As well as precision Cv dosage <2% for multiple laboratory practices.
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