Polymerase Chain Reaction Functions for COVID-19 Detection

November 18, 2022by Kalstein
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The Polymerase Chain Reaction (PCR) is a laboratory test that aids in the reproduction or amplification of billions of copies of a specific DNA segment, so that it can be studied in detail. PCR uses short pieces of synthetic DNA, called primers, to choose a segment of the genome that will reproduce (amplify) and then use multiple sessions of DNA synthesis to amplify that segment.

Immediately after amplification, the DNA obtained by PCR is used for various laboratory procedures and clinical techniques, such as mapping the human genome project, forensic analysis, paternity testing, diagnosis of genetic disorders, detection of microorganisms such as salmonella, AIDS, hepatitis, or COVID-19.

The Polymerase Chain Reaction technique has long been used in laboratories in various health crises in relation to infectious diseases, however, because of the COVID-19 pandemic, this test has been extended and made even more known. According to the World Health Organization, PCR is considered the most reliable diagnostic test that determines whether or not a patient has COVID-19.

Polymerase Chain Reaction for COVID-19 detection

The Polymerase Chain Reaction (PCR) test is used for the diagnosis of the COVID-19 virus in patients, because it allows the detection of a fragment of genetic material of the pathogen. This test has become an essential piece of simple and rapid diagnosis of infectious diseases.

The virus that causes COVID-19 is SARS-CoV-2, which is part of the Coronavirus family. The PCR test is used by professionals to diagnose this virus, by collecting a sample of nasal and oral fluid, using a long or short swab, depending on whether the sample will be taken from the nose or throat, in this way it can detect the presence of the virus genetic material (RNA).

Professionals advise that the sample be taken between the third and seventh day after having symptoms from COVID-19, since that time period is the period of infection where there is greater viral activity, making it easier to find the virus in the sample taken from the patient. Finally, test results may be available in a few minutes or about 1 to 3 days, depending on the laboratory in which the test is done.

PCR Thermocycler

The thermocycler, also known as a thermal sequencer, is a laboratory apparatus that allows amplification of DNA molecules through the technique of โ€œPolymerase Chain Reactionโ€ or PCR. It works by cycles of temperatures through which the amplification of this nucleic acid occurs. PCR allows a small amount of DNA molecules to be amplified many times, this technique is an automatic process that consists of a few hours and is carried out by the thermo-cycler, which is programmed to change the reaction temperature in each of the cycles to which the sample containing DNA is subjected.

A conventional thermo-cycler consists of an electrical resistance block that, by means of a plate, allows the temperature to increase and remain homogeneous during the cycles that the PCR test lasts. This equipment can be programmed and usually works at a temperature between 4ยฐ C and 96ยฐ C. It has its application in various areas, such as medicine, paleontology, forensic sciences and biology, among others.

Kalstein brand PCR thermocycler

At Kalstein, we have a high range of useful laboratory equipment in the area of science and medicine, our thermocyclers are of high quality and technology, this is assured because we are MANUFACTURERS of them.ย  In addition to quality, we have the best PRICES in the market. If you are interested in making the PURCHASE of some of our thermo-cyclers available for sale, you can find a variety of models in our catalog, by visiting the following link HERE. Our real-time PCR YR series has the following characteristics:

  • Innovative Optical Design
  • Two-channel (YR01869-1/2) and five-channel (YR01869-1/2) fluorescent detection system with high-resolution CCD and LED light source
  • The optical system automatically collects data from all wells during data acquisition at the same time.

For more information about our other products, see HERE

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