Power Sources for Electrophoresis Runs

The electrophoresis power source potency is designed for use in DNA / RNA separation, RFLP, DNA fragmentation, polyacrylamide gel electrophoresis, and protein separation. Electrophoresis is a method by which molecules are separated by size or molecular weight using an electrical current.

The separation is commonly done on a gel that functions as a filter and is usually made up of materials such as agarose or polyacrylamide. Its main use is the separation of mixtures of macromolecules, especially nucleic acids (DNA or RNA) or proteins.

By placing a sample of proteins inside the gel and passing an electrical current, the proteins will move through the gel towards the positive pole. Smaller molecules will move faster and larger molecules will stay close to where they started in the gel.

Electrophoresis and Gel Documentation Systems

Gel electrophoresis and the gel documentation system are techniques that separate molecules according to size and electrical charge, they are mostly used in laboratories to separate macromolecules such as DNA, RNA, and proteins.

Electrophoresis works using a gel, made of a material called agarose, it is gelationous and created with seaweed, it is porous and thanks to it, macromolecules of many different sizes are separated, due to the gel being immersed in a buffer solution of the salt in an electrophoresis chamber.

The chamber has two electrodes - one positive and one negative - at its two ends. The samples that need to be analyzed are then loaded into tiny wells on the gel with the help of a pipette.

What is electrophoresis?

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size to electric charge ratio, using a gelatinous matrix as a base. When a mixture of ionized and net charged molecules are positioned in an electric field; These experience a force of attraction towards the pole that has an opposite charge; Allowing a certain time to elapse, the positively charged molecules will move towards the cathode (negative pole) and those positively charged will move towards the anode (positive pole).

Gel electrophoresis is used in laboratories to classify and measure proteins or nucleic acids.

When did this technique begin to be used?

Electrophoresis began in earnest with the work of Arne Tiselius in 1931, and new separation processes and chemical analysis techniques based on electrophoresis continue to be developed in the 21st century.

Types of Electrophoresis Systems

Horizontal Electrophoresis

Horizontal gel electrophoresis uses the basic theory for the separation of DNA, RNA or protein molecules according to their respective molecular size and charge. In this technique, the gel is present in a horizontal orientation and is immersed in a buffer that is continuous. The agarose gel is used to separate the gel box into two compartments.

Vertical Electrophoresis

The vertical gel electrophoresis technique works according to the primary theory of gel electrophoresis, but is considered to be more complex than the horizontal gel electrophoresis method. This technique uses a discontinuous buffer. A cathode is located in the upper chamber, and the anode is located in the lower chamber. The electrodes present in each compartment provide the required electric field.

KALSTEIN ELECTROPHORESIS SYSTEMS

At Kalstein you can find the ideal electrophoresis systems for your laboratory.

Electrophoresis is a common technique in the clinical laboratory. It allows the separation of chemical species (nucleic acids or proteins) along an electric field based on their size and their electric charge.

Capillary Electrophoresis

It is a tool for separating biomolecules, which in recent years has had great importance in medicine; It has the versatility of being able to separate amino acids, organic acids, inorganic ions, carbohydrates, steroids, among others.

Agarose Gel Electrophoresis

It is a molecular biology method for analyzing and separating DNA fragments based on their size. When you use gel electrophoresis to assist you in your cloning experiments, you may encounter very common problems.

Polyacrylamide Gel Electrophoresis

Polyacrylamide is produced as a result of the polymerization reaction between acrylamide and N, N'-methylene-bis-acrylamide (BIS) using a catalyst. The degree of polymerization or interconnection can be controlled by adjusting the concentration of acrylamide and BIS.

Our Best Selling Electrophoresis System

YR03425 Mini Protean Vertical Electrophoresis Cell
Product description

Vertical gel electrophoresis is a more complex setup compared to horizontal gel system. Researchers often use this system to separate proteins instead of nucleic acids. However, before separating the proteins, it is necessary to break the quaternary structure of the proteins at the linear strand.

Particularity
Installing the glass plate in just 15 seconds, and you don't need the button, it's quick and convenient.
With a gel mold in its original position, it is not necessary to move the vessels from gel fabrication to electrophoresis. It is more convenient to observe the gel preparation on both sides of the vessels.

KALSTEIN UPDATED

What is your ideal laboratory Electrophoresis System?

There are countless models, so it is normal that you do not know which laboratory electrophoresis to buy that suits your needs. At Kalstein, we test them to find what you're looking for.

Horizontal and vertical electrophoresis

Gel electrophoresis is a laboratory technique used in genetics to separate mixtures containing DNA, RNA and other proteins according to their respective molecular size and charge.

Hemoglobin electrophoresis and hemoglobinopathies

In a generic way, the clinical syndromes that result from alterations in hemoglobin (Hb) are known as hemoglobinopathies and to know them is electrophoresis

What is laboratory electrophoresis?

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size-to-electric charge ratio.

Multiple Myeloma and Protein Electrophoresis

Multiple Myeloma; is a multifocal plasma cell neoplasm that affects the bone marrow and is associated with the production of a serum and urinary monoclonal protein. The cause is a progressive unregulated proliferation of plasma cells that accumulate in the bone marrow. These cells secrete excess immunoglobulin (Ig), usually: IgG 57%; IgA 21%, IgD 1%, IgM, IgE, only rarely in 18% of cases of light chains alone. The proliferation of multiple myeloma interferes with normal cell production in the bone marrow and usually results in anemia.

Electrophoresis Systems on Offer

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