A micropipette is a laboratory instrument used to absorb and transfer small volumes of liquids, and to allow their handling in different laboratory techniques.
The volumes that can be captured by these devices vary depending on the model and type of micropipette: the most common ones, called p20, p200, and p1000, support a maximum of 20, 200, and 1000, respectively. Micropipettes are very useful for handling any kind of liquid because their tip can be discarded, as they use disposable tips, plastic, which are usually sterile. A micropipette is made up of a push-button or plunger, sucker, and dispenser of the liquid.
Types of micropipettes
Micropipettes are useful for handling any type of liquid, because their tip can be discarded, since they use disposable plastic tips. Within the category of micropipettes can be found:
- Fixed volume micropipettes: They are those micropipettes that do not allow to adjust the volume, but are already configured to take a fixed volume of the liquid to be manipulated, which makes them highly precise micropipettes in the measurements they make.
- Variable volume micropipettes: Variable volume micropipettes are the ideal precision instrument for liquid measurement needs in clinical laboratory and quality control. The volumes that can be measured by these instruments vary according to the model and type of pipette: the most common ones, called p20, p200, and p1000, support a maximum of 20, 200, and 1000, respectively. Being able to measure a volume range that the device allows according to its accuracy, i.e. with a p20 I can also measure a volume of 15 □ l but not more than 20 □ l.
- Multi-channel micropipettes: A multichannel micropipette as its name suggests is a micropipette that has several channels, its operation is similar to that of single-channel micropipettes, through air displacement. Depending on the model can be 8 to 12 channels. Allow all channels to be operated at once. Dramatically saving the execution time of a technique. These can be fixed volume or they can also allow you to select the desired volume.
Recommendations for the correct use of a micropipette
In order to use a micropipette correctly, it must be taken into consideration that it has three fundamental steps in general. As are rest or rest, the first stop and the second stop. It is also convenient to have basic notions of how to pipette correctly with a micropipette:
- Never rotate the volume indicator dial beyond the upper or lower limit of the micropipette.
- The tips are one of the most important parts of the micropipette, it is necessary to make sure that they are the correct size and are always sterile.
- Pipetting is more accurate when the instrument is held at an angle of less than 2000̊2 vertical.
- When releasing the liquid, it is recommended to support the tip of the micropipette slightly against the test tube, so the surface tension helps to dispense the contents.
- A common operator error is to push the plunger to the second stop before filling the tip of the micropipette.
- It is important to verify that the tip fits with minimum effort and that it comes out with the same ease; it is always advisable to check the micropipette manual to avoid making mistakes.
What do we offer you in Kalstein?
At Kalstein we are MANUFACTURERS of laboratory equipment so we make available novel micropipettes at the best PRICES on the market. This time we present our Multichannel Liquid Handling Pipette YR03737, designed and manufactured to offer you a range of robust, accurate and precise pipettes. This micropipette has the following characteristics:
- 3 digits.
- Form of increase.
- Chemical resistance.
- Sterilization at 121 °C.
- Operating force very low.
- The color indicates the volume of the pipette.
- Placed for perfect ergonomics.
- Only a few turns to reach the desired volume.
For more information we invite you to take a look at HERE