Real-time PCR System YR06248 // YR06249

The Real-Time Quantitative PCR System YR06248 – YR06249 is a highly advanced tool designed for precise and sensitive PCR analysis. It features cutting-edge signal capture and spectrum separation technology, ensuring excellent correction of multicolor crosstalk and consistent temperature uniformity. With a temperature range from 4°C to 100°C, and a Peltier-based heating and cooling system, it guarantees reliable results and reproducibility. This PCR system offers both 4-channel and 5-channel configurations, making it ideal for multiplex PCR applications. It supports up to 4 targets simultaneously, or 5 targets with the 5-channel model. Compatible with a wide range of qPCR dyes and probes, including FAM, VIC, ROX, and Cy5, it is versatile enough for various lab needs. Its dynamic range spans up to 10 orders of magnitude, and it can detect as low as a single gene copy.

Market Price

The price of the YR06248 – YR06249 Real-Time Quantitative PCR System typically ranges from USD $19,950 to USD $21,610. This price varies based on the selected model and configuration. For an accurate quote, you can request a quotation through Kalstein Plus and enjoy exclusive discounts as a Kalstein+ member.

Frequently Asked Questions

• What is the sensitivity of the YR06248 PCR system? The system can detect as low as one gene copy, making it suitable for low-concentration samples.
• Is the system compatible with other PCR reagents? Yes, it works with a variety of dyes and probes, including FAM, SYBR Green, VIC, ROX, and Cy5, offering flexibility for multiplex assays.
• What maintenance does the system require? Regular maintenance includes cleaning optical components, calibrating temperature controls, and updating software for optimal performance.

Advantages and Disadvantages

Advantages:

• High sensitivity, capable of detecting a single gene copy.
• Compatible with a wide range of dyes and probes.
• Supports multiplexing up to 4 targets (5 for the 5-channel model).
• High resolution and accuracy, even with complex samples.
• Precision heating and cooling with Peltier technology.

Disadvantages:

• High cost, which may be a concern for labs with limited budgets.
• Its size could be a challenge in smaller laboratory spaces.

Real-World Use

The YR06248 – YR06249 PCR system is primarily used in genetic research labs, molecular diagnostics, and pharmaceutical studies. It is invaluable for DNA and RNA quantification, gene expression analysis, genotyping, and genetic variant studies. Clinically, it is used for diagnosing infectious diseases, cancer, and genetic disorders. The system’s multiplex PCR capability enables efficient analysis of multiple biomarkers in one run, saving both time and resources in high-demand labs.

Recommendations

To achieve optimal results, use high-quality reagents that are compatible with the system. Regular calibration of temperature and optical systems is crucial. Labs dealing with low-concentration or complex samples will particularly benefit from the system’s high precision and dynamic range.

Technical Specifications

Model	YR06248	YR06249
Sample capacity	8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate
Sample volume	10-50 μL
Heating and Cooling method	Peltier
Maximum ramping rate	6.0° C/s
Temperature range	4-100 °C
Temperature accuracy	± 0.2°C
Temperature uniformity	±0.2°C@60°C ±0.3°C@95°C
Optical Detecting System
Excitation light	4pcs high-efficiency single color LED	5pcs high-efficiency single color LED
Detector	SiPMT
Detection method	Time-resolved real-time scanning
Excitation/Emission wavelengths	455-650nm	510-750nm
Detection Channels	4 (optional 5 channels）
Supported dyes	FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model）
Multiplexing	Up to 4 targets (optional 5 targets for 5-channel model)
Sensitivity	1 copy gene
Resolution	1.33-fold copies difference in single-plex reaction
Dynamic range	10 orders of magnitude