Gel Documentation

A gel photo documentation system is a composite system used to observe, take photos and analyze the bands of the gels obtained from electrophoretic runs. These systems are designed to allow clear, high-quality images to be obtained, offering better analysis, and providing a results backup system, which is essential when publishing the results of an investigation.

These equipments allow the reading of the results in a fast and precise way, they reduce the probabilities of human error when analyzing and quantifying the results, because it allows us to observe those bands that are not observed with the naked eye, such as those observed by fluorescence, thanks to the presence of a series of specialized elements that allow the image to be captured.

Electrophoresis is one of the main molecular biology techniques, they are considered the most used technique in laboratories in everything related to nucleic acid research. Using electrophoresis, we can separate DNA and RNA fragments based on their size and electrical charge through their migration through a porous material (gel), visualize them by means of a staining, many of them based on fluorescence, such as ethidium bromide. , and determine the content of nucleic acids or proteins in a sample, thus having an estimate of their concentration.

What is a fluorescence gel photo documentation system?

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Our best-selling Gel Documentation System:

Characteristics

Specifications

Characteristics

Gel system YR04961 - YR04962

The Gel Documentation and Analysis System is designed for the detection and documentation of nucleic acids and proteins. It adopts a high-resolution and high-sensitivity scientific CCD camera, which enables the instrument to capture absolutely weak signals under extremely low lighting conditions. The automatic controls and intuitive software that we offer can help researchers get rid of the complicated operating process and thus increase the efficiency of the experiment.

Gel inspection

Various dyes, for example: Ethiduim Bromide, SYBR ™ Gold, SYBR ™ Green, SYBR ™ Safe, GelStar ™, Texas Red, Fluorescein, tagged DNA / RNA detection.

Specifications

RIM Molding Polymer

The front panel and door are designed by Reaction Injection Molding. It is friendly with the environment.

Upgrade easily

Select a configuration for your applications now and update later when applying different applications by adding another tray or hardware module:

a) UV Transmission

b) UV + BLUE Light transmission

c) UV + BLUE + White light transmission

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Considerations for the use of the Gel Documentation System:

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The gel used in gel electrophoresis is generally made from a material called agarose, which is a gelatinous substance extracted from seaweed. This porous gel could be used to separate macromolecules of many different sizes.

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The gel is immersed in a buffer solution of the salt in an electrophoresis chamber. Tris-borate-EDTA (TBE) is commonly used as the buffer. Its main function is to control the pH of the system. The chamber has electrodes, one positive and one negative

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The samples that need to be analyzed are then loaded into tiny wells on the gel with the help of a pipette. Once the load is ready, an electric current of 50-150 V is applied. Now, the molecules in the sample start towards the electrodes.

The speed at which each molecule travels through the gel is called its electrophoretic mobility and is primarily determined by its net charge and size. Molecules that are strongly charged begin to move faster than those that are weakly charged.

Once the separation is complete, the gel is stained with a dye to reveal the bands from the separation. The gel is soaked in a dilute ethidium bromide solution and then placed on an ultraviolet transilluminator to visualize the bands.

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What is your Laboratory Gel Documentation System?

There are countless models, so it is normal that you do not know which Laboratory Gels Documentation System to buy that suits your needs. At Kalstein, we test them to find what you're looking for.

What is a fluorescence gel photo documentation system?

Electrophoresis and Gel Documentation Systems

Gel electrophoresis is a widely used technique in life science laboratories to separate macromolecules such as DNA, RNA, and proteins. In this technique, molecules are separated according to size and electrical charge.

The Documentation System in Laboratory Gels

The choice of a gel documentation system depends on your current and anticipated future needs. Bio-Rad Gel Documentation Systems can accommodate a wide range of samples ...

Electrophoresis

Electrophoresis is an analytical method in which a controlled electric current is used in order to separate biomolecules according to their size to electric charge ratio, using a gelatinous matrix as a base.

Electrophoresis is used in a great majority in the field of recombinant DNA since it allows us to know the charge that polypeptides have, and to separate the different polypeptides resulting from the variations of the recombinant DNA experiment.